IVF Procedures
Correction table to maintain the same partial pressure in incubator of CO2 and O2 as at sea level
EXAMPLE:
Laboratory is at 1500 m above sea level where the partial pressure is 0,85 atm. The desired CO2 concentrati...
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Calculation of sperm suspension volume to use for fertilizing in 100μl drops
The dilution is 1:2 as 25 µl of sperm suspension has been mixed with 25 µl of cold waterAnd the number of sperm count...
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Culture conditions
Elevate bovine IVF success rates with Dr. Lotte Stroebech’s comprehensive guidelines, emphasizing precise temperature control, optimal gas concentrations, and proper media handling for consistent embryo development.
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Day-1: Maturation IVM
Streamline oocyte handling and transport for Ovum Pick-Up (OPU) and in vitro maturation (IVM) with Dr. Lotte Stroebech’s proven guidelines, ensuring optimal temperatures, media equilibration, and best practices to boost IVF success rates.
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Day 0: Fertilization IVF
Optimize Day 0 fertilization in bovine IVF with Dr. Lotte Stroebech’s proven protocols for proper media preparation, oocyte handling, and precise semen processing, ensuring enhanced efficiency and reliability in embryo production.
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Day 1: Culture IVC
Maximize bovine IVF success on Day 1 (Culture IVC) using Dr. Lotte Stroebech’s advanced protocols for precise media preparation, efficient denudation, and meticulous oocyte tracking—ensuring optimal embryo development.
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Correlation between Bicarbonate and CO2 Concentrations and the effect on pH
• pH will be 7.35 in a medium with a concentration of 2.1 mg/ml bicarbonate at 6% CO2• Lower CO2 = increase pH• Know...
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