Zymo-Seq RRBS Library Kit
Zymo-Seq RRBS Library Kit is an all-in-one RRBS library prep kit that generates NGS libraries with a simplified workflow for the classic method Reduced Representation Bisulfite Sequencing (RRBS). This all-in-one RRBS library prep kit allows for DNA methylation profiling at single-nucleotide resolution in CpG-rich regions of the genome. This Zymo-Seq RRBS Library Kit is the ideal RRBS library prep kit to generate libraries that cover a significant percent (=70% if using human genomic DNA) of all CpG islands, promotors, and gene bodies while largely reduces the sequencing cost, making it ideal for high-throughput screening and comparison experiments. Now includes Unique Dual Index (UDI) Primer Sets.
Specifics
| Equipment Required | Microcentrifuge, thermocycler with a heated lid |
| Input | 10 – 500 ng of genomic DNA |
| Sample Sources | Purified genomic DNA. The DNA should be free of enzymatic inhibitors and can be suspended in water, TE, or a low salt buffer. DNA with low 260/280 or 260/230 ratios should be purified prior to processing using the Genomic DNA Clean & Concentrator (Cat. No. D4010). This protocol is not recommended for samples from species with low CpG density. |
| Sequencing Platform Compatible | Libraries are compatible with all Illumina sequencing platforms except HiSeq X Series. |
