{"product_id":"zymo-seq-wgbs-library-kit","title":"Zymo-Seq WGBS Library Kit","description":"\u003ch1 id=\"ctl00_ctl00_ctl00_BodyContent_BodyContent_BodyContent_ctl00_ptPageTitle_h1Heading\" class=\"heading heading--1\" data-source=\"u-miscellaneous-pagetitle\" data-search-term-container=\"\"\u003e\u003cspan id=\"ctl00_ctl00_ctl00_BodyContent_BodyContent_BodyContent_ctl00_ptPageTitle_spanItemProp\"\u003e\u003cspan class=\"highlight\"\u003e\u003cspan class=\"highlight__text\"\u003eZymo\u003c\/span\u003e-Seq WGBS Library\u003cspan\u003e \u003c\/span\u003e\u003cspan class=\"highlight__text\"\u003eKit\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/h1\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cspan class=\"highlight\"\u003e\u003cspan class=\"highlight__text\"\u003eZymo\u003c\/span\u003e-Seq WGBS Library\u003cspan\u003e \u003c\/span\u003e\u003cspan class=\"highlight__text\"\u003eKit\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003eis the only kit available for whole genome bisulfite (WGBS) library preparation in a single tube. Zymo-Seq WGBS Library Kit incorporates tagmentation technology to eliminate the tedious fragmentation, enzymatic, and clean-up steps required by conventional, ligation-based library preparation methods. This streamlined workflow minimizes hands-on time, making it an ideal choice for higher throughput applications. To prepare Zymo-Seq WGBS libraries, intact genomic DNA is first bisulfite converted. Then, the following library preparation procedures are completed in a single tube: (1) second-strand synthesis, (2) adapterization via tagmentation, and (3) library amplification and indexing. After purification, libraries are ready for sequencing on Illumina instruments.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cbr data-mce-fragment=\"1\"\u003e\u003cspan class=\"highlight\"\u003eWGBS is the gold standard for DNA methylation studies as it provides base-level methylation quantification for all cytosines.\u003cspan\u003e \u003c\/span\u003e\u003cspan class=\"highlight__text\"\u003eZymo\u003c\/span\u003e-Seq WGBS Library\u003cspan\u003e \u003c\/span\u003e\u003cspan class=\"highlight__text\"\u003eKit\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003eis ideal for any species for whole-genome methylation calling of CG, CHG, and CHH sites. Over 90% of human and mouse CpG sites can be detected from Zymo-Seq libraries.\u003c\/span\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli class=\"text-secondary list-unstyled mb-3 pr-2\"\u003eWhole Genome Bisulfite (WGBS) library preparation in less than 4 hours and in one tube.\u003c\/li\u003e\n\u003cli class=\"text-secondary list-unstyled mb-3 pr-2\"\u003eReduced library preparation bias for accurate methylation calling\u003c\/li\u003e\n\u003cli class=\"text-secondary list-unstyled mb-3 pr-2\"\u003eReproducible genome coverage from any species (\u0026gt;90% of CpG sites detected in mammalian samples)\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003eDNA Input\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eFor optimal results, use 100 ng of high quality, intact genomic DNA as input. Protocol is compatible with inputs between 10 ng to 100 ng. DNA should be free of enzymatic inhibitors and RNA contamination. DNA can be resuspended in water, TE, or a low salt buffer.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eProcessing Time\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e~ 4 hours\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eRequired Equipment\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eMicrocentrifuge, thermal cycler with heated lid, magnetic separator, multichannel pipette (suggested)\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eRequired material not provided\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eIllumina DNA Prep Kit (Illumina, Cat. No. 20018704 or 20018705)\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSequencing Platform\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eLibraries are compatible with all Illumina sequencing platforms (MiSeq, MiniSeq, HiSeq, NextSeq, NovaSeq)\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/files.zymoresearch.com\/protocols\/d5465-zymo_seq_wgbs_library_kit.pdf\" target=\"_blank\" title=\"Zymo-Seq WGBS Library Kit Protocol\" rel=\"noopener\"\u003e\u003cspan\u003e\u003cspan class=\"highlight\"\u003eProtocol \u003c\/span\u003e\u003c\/span\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/files.zymoresearch.com\/datasheets\/ds2705-zymo_seq_wgbs_library_kit.pdf\" target=\"_blank\" title=\"Zymo-Seq WGBS Library Kit Datasheet\" rel=\"noopener\"\u003e\u003cspan\u003e\u003cspan class=\"highlight\"\u003eDatasheet \u003c\/span\u003e\u003c\/span\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/files.zymoresearch.com\/sds\/sdd5465-zymo_seq_wgbs_library_kit-us.pdf\" target=\"_blank\" title=\"Zymo-Seq WGBS Library Kit SDS\" rel=\"noopener\"\u003e\u003cspan\u003e\u003cspan class=\"highlight\"\u003eSDS (MSDS)\u003c\/span\u003e\u003c\/span\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"Zymo Research","offers":[{"title":"1 EA","offer_id":44879073607834,"sku":"D5465","price":1185.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0593\/5025\/3722\/files\/d5465-zymo_seq_wgbs_library_kit_600x_ddb91a3a-298a-44ae-954e-cf57730e7dc6.webp?v=1715781551","url":"https:\/\/animal.ivfstore.com\/es\/products\/zymo-seq-wgbs-library-kit","provider":"Animal IVF Store","version":"1.0","type":"link"}