{"product_id":"zymo-seq-rrbs-library-kit","title":"Zymo-Seq RRBS Library Kit","description":"\u003ch2 id=\"ctl00_ctl00_ctl00_BodyContent_BodyContent_BodyContent_ctl00_ptPageTitle_h1Heading\" class=\"heading heading--1\" data-source=\"u-miscellaneous-pagetitle\"\u003e\u003cspan id=\"ctl00_ctl00_ctl00_BodyContent_BodyContent_BodyContent_ctl00_ptPageTitle_spanItemProp\"\u003e\u003cspan class=\"highlight\"\u003e\u003cspan class=\"highlight__text\"\u003eZymo\u003c\/span\u003e-Seq RRBS Library\u003cspan\u003e \u003c\/span\u003e\u003cspan class=\"highlight__text\"\u003eKit\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003e\u003cmeta charset=\"utf-8\"\u003e \u003cspan class=\"highlight__text\"\u003eZymo\u003c\/span\u003e\u003cspan class=\"highlight\"\u003e-Seq RRBS Library \u003cspan class=\"highlight__text\"\u003eKit\u003c\/span\u003e is an all-in-one RRBS library prep kit that generates NGS libraries with a simplified workflow for the classic method Reduced Representation Bisulfite Sequencing (RRBS). This all-in-one RRBS library prep kit allows for DNA methylation profiling at single-nucleotide resolution in CpG-rich regions of the genome. This Zymo-Seq RRBS Library Kit is the ideal RRBS library prep kit to generate libraries that cover a significant percent (=70% if using human genomic DNA) of all CpG islands, promotors, and gene bodies while largely reduces the sequencing cost, making it ideal for high-throughput screening and comparison experiments.  Now includes Unique Dual Index (UDI) Primer Sets.\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch4\u003e\u003cspan\u003e\u003cspan class=\"highlight\"\u003eSpecifics\u003c\/span\u003e\u003c\/span\u003e\u003c\/h4\u003e\n\u003ctable class=\"table spec-table\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eEquipment Required\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003eMicrocentrifuge, thermocycler with a heated lid\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eInput\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e10 – 500 ng of genomic DNA\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSample Sources\u003c\/td\u003e\n\u003ctd\u003e\n\u003cspan\u003ePurified genomic DNA. The DNA should be free of enzymatic inhibitors and can be suspended in water, TE, or a low salt buffer. DNA with low 260\/280 or 260\/230 ratios should be purified prior to processing using the \u003c\/span\u003e\u003cstrong\u003eGenomic DNA Clean \u0026amp; Concentrator (Cat. No. D4010)\u003c\/strong\u003e\u003cspan\u003e. This protocol is not recommended for samples from species with low CpG density.\u003c\/span\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSequencing Platform Compatible\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003eLibraries are compatible with all Illumina sequencing platforms except HiSeq X Series.\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan class=\"highlight\"\u003e\u003ca href=\"https:\/\/files.zymoresearch.com\/protocols\/d5460_d5461_zymo-seq_rrbs_library_kit.pdf\" target=\"_blank\" title=\"Zymo-Seq RRBS Library Kit Protocol\" rel=\"noopener\"\u003eProtocol\u003c\/a\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/files.zymoresearch.com\/datasheets\/ds2702_zymo-seq_rrbs_library_kit.pdf\" target=\"_blank\" title=\"Zymo-Seq RRBS Library Kit Datasheet\" rel=\"noopener\"\u003e\u003cspan\u003e\u003cspan class=\"highlight\"\u003eDatasheet \u003c\/span\u003e\u003c\/span\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/files.zymoresearch.com\/sds\/d5460_d5461-ez_dna_methylation_rrbs_library_prep_kit.pdf\" target=\"_blank\" title=\"Zymo-Seq RRBS Library Kit SDS\" rel=\"noopener\"\u003e\u003cspan\u003e\u003cspan class=\"highlight\"\u003eSDS (MSDS)\u003c\/span\u003e\u003c\/span\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"Zymo Research","offers":[{"title":"Pack of 48 Preps","offer_id":44880487481498,"sku":"D5461","price":3210.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0593\/5025\/3722\/files\/d5460_ez_dna_methylation_rrbs_library_prep_kit_600x_031818c7-f06e-48d5-ad44-9a96c6c39c9d.webp?v=1715782403","url":"https:\/\/animal.ivfstore.com\/es\/products\/zymo-seq-rrbs-library-kit","provider":"Animal IVF Store","version":"1.0","type":"link"}