{"product_id":"zymo-seq-ribofree-universal-cdna-kit","title":"Zymo-Seq RiboFree Universal cDNA Kit","description":"\u003ch1 id=\"ctl00_ctl00_ctl00_BodyContent_BodyContent_BodyContent_ctl00_ptPageTitle_h1Heading\" class=\"heading heading--1\" data-source=\"u-miscellaneous-pagetitle\" data-search-term-container=\"\"\u003e\u003cspan id=\"ctl00_ctl00_ctl00_BodyContent_BodyContent_BodyContent_ctl00_ptPageTitle_spanItemProp\"\u003e\u003cspan class=\"highlight\"\u003e\u003cspan class=\"highlight__text\"\u003eZymo\u003c\/span\u003e-Seq RiboFree Universal cDNA\u003cspan\u003e \u003c\/span\u003e\u003cspan class=\"highlight__text\"\u003eKit\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/h1\u003e\n\u003ch4\u003eDescription\u003c\/h4\u003e\n\u003cp\u003e\u003cspan class=\"highlight\"\u003eThe\u003cspan\u003e \u003c\/span\u003e\u003cspan class=\"highlight__text\"\u003eZymo\u003c\/span\u003e-Seq RiboFree Universal cDNA\u003cspan\u003e \u003c\/span\u003e\u003cspan class=\"highlight__text\"\u003eKit\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003eis the simplest kit for the preparation of NGS-grade first-strand cDNA with ribosomal RNA (rRNA) depleted. This simple cDNA kit features RiboFree Universal Depletion, a novel probe-free rRNA depletion technology that depletes rRNA from any organism. The RiboFree Universal Depletion is validated across biological kingdom and phyla, including mammals, plants, and various prokaryotes, and also RNA from a wide range of sample types including cells, snap-frozen tissues, and FFPE samples. The RiboFree single-stranded cDNA produced by the kit can be used for qPCR detection, RNA-Seq library prep, and any other downstream analysis. In contrast to poly(A) enrichment methods, the RiboFree Universal cDNA Kit produces single-stranded cDNA from nearly all RNA biotypes, including full-length mRNA, mRNA with degraded poly(A) tails, long-noncoding RNA (lncRNA), intronic RNA, nucleolar RNA, etc. The cost-effective Zymo-Seq RiboFree Universal cDNA Kit has been optimized to reduce hands-on time, with all the reagents such as SPRI beads included for researchers’ convenience.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch4\u003eHighlights\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eUniversal Depletion: Novel probe-free technology depletes rRNA from any organism.\u003c\/li\u003e\n\u003cli\u003e\u003cspan class=\"highlight\"\u003eSimplest\u003cspan\u003e \u003c\/span\u003e\u003cspan class=\"highlight__text\"\u003eKit\u003c\/span\u003e: Prepare NGS-grade RiboFree cDNA from total RNA in as little as 1.75 hours.\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003eAutomation Friendly: Streamlined protocol for increased scalability.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003eTable 2. Zymo-Seq RiboFree Universal cDNA Kit (Cat. No. R3001) update summary\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable border=\"1\" cellpadding=\"0\" cellspacing=\"1\" width=\"600\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e \u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eCurrent Version\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eUpdated Version\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eInstruction Manual Versions\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003ev1.1.0\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003ev2.0.0\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eTotal RNA Input Range\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e100 ng – 5 µg\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e10 ng – 1000 ng\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eDepletion Reaction Duration*\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e100 ng input – 4 hours\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e· 100 ng input – 1 hour\u003cbr\u003e· 10 ng input – 4 hours\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cem\u003e* May vary depending on the quantity, quality, and source of the input RNA.\u003c\/em\u003e\u003c\/p\u003e\n\u003ctable border=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eEquipment Needed (user provided)\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003eThermal cycler with heated lid, magnetic stand for 0.2 mL PCR tubes, microcentrifuge for 0.2 mL PCR tubes and 1.5 mL microcentrifuge tubes, and a benchtop vortex mixer.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eSample Input Material\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003eRNA\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eRNA Input\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e10 – 1000 ng of total RNA.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eFor optimal results, please use the recommended 10-1000 ng input.\u003c\/li\u003e\n\u003cli\u003eIf an input below 10 ng is necessary, see\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003eAppendix B\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eof the kit protocol for additional considerations and recommended modifications.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eInput Quality\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003eRNA should be free of DNA contamination and enzymatic inhibitors, with A260\/A280 and A260\/A230\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003e≥ 1.8\u003c\/strong\u003e. RNA with lower purity ratios (A260\/A280 and A260\/A230) should be treated with DNase I and purified with the\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003eRNA Clean \u0026amp; Concentrator™ (Cat. No. R1013)\u003cspan\u003e \u003c\/span\u003e\u003c\/strong\u003eprior to processing. RNA should be suspended in water, TE, or a low-salt buffer.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eFor optimal results, please use intact RNA (RNA Integrity Number or RIN ≥ 8.0) whenever possible.\u003c\/li\u003e\n\u003cli\u003eFor degraded RNA input, see\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003eAppendix C\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eof the protocol for additional considerations and recommended modifications.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eProcessing Time\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003eAs little as 1.75 hours\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan class=\"highlight\"\u003e\u003ca href=\"https:\/\/files.zymoresearch.com\/protocols\/r3001_zymo-seq_ribofree_universal_cdna_kit.pdf\" target=\"_blank\" title=\"Zymo-Seq RiboFree Universal cDNA Kit Protocol\" rel=\"noopener\"\u003eProtocol\u003c\/a\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/files.zymoresearch.com\/datasheets\/ds2703_02_zymo-seq_ribofree_universal_cdna_kit_data_sheet.pdf\" target=\"_blank\" title=\"Zymo-Seq RiboFree Universal cDNA Kit Datasheet\" rel=\"noopener\"\u003e\u003cspan\u003e\u003cspan class=\"highlight\"\u003eDatasheet \u003c\/span\u003e\u003c\/span\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/files.zymoresearch.com\/sds\/sdr3001_-_zymo-seq_ribofree_universal_cdna_kit_(US).pdf\" title=\"Zymo-Seq RiboFree Universal cDNA Kit SDS - R3001\" rel=\"noopener\" target=\"_blank\"\u003e\u003cspan\u003e\u003cspan class=\"highlight\"\u003eSDS (MSDS)\u003c\/span\u003e\u003c\/span\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"Zymo Research","offers":[{"title":"Pack of 12 Preps","offer_id":44880577462426,"sku":"R3001","price":706.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0593\/5025\/3722\/files\/Zymo-SeqRiboFreeUniversalcDNAKit_Custom_600x_7b4480bd-e01a-40ba-be48-9a2ded77d9e3.webp?v=1715784603","url":"https:\/\/animal.ivfstore.com\/es\/products\/zymo-seq-ribofree-universal-cdna-kit","provider":"Animal IVF Store","version":"1.0","type":"link"}