{"product_id":"zymo-seq-ribofree-total-rna-library-kit","title":"Zymo-Seq RiboFree Total RNA Library Kit","description":"\u003ch2 id=\"ctl00_ctl00_ctl00_BodyContent_BodyContent_BodyContent_ctl00_ptPageTitle_h1Heading\" class=\"heading heading--1\" data-source=\"u-miscellaneous-pagetitle\"\u003e\u003cspan id=\"ctl00_ctl00_ctl00_BodyContent_BodyContent_BodyContent_ctl00_ptPageTitle_spanItemProp\"\u003e\u003cspan class=\"highlight\"\u003e\u003cspan class=\"highlight__text\"\u003eZymo\u003c\/span\u003e-Seq RiboFree Total RNA Library\u003cspan\u003e \u003c\/span\u003e\u003cspan class=\"highlight__text\"\u003eKit\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/h2\u003e\n\u003ch4\u003eDescription\u003c\/h4\u003e\n\u003cp\u003e\u003cspan class=\"highlight\"\u003e\u003cspan class=\"highlight__text\"\u003eZymo\u003c\/span\u003e-Seq RiboFree Total RNA Library\u003cspan\u003e \u003c\/span\u003e\u003cspan class=\"highlight__text\"\u003eKit\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003eis the simplest RNA-Seq library prep kit available to make stranded, ribosomal RNA (rRNA) depleted, total RNA libraries. This all-inclusive RNA-Seq library prep kit features a novel probe-free rRNA depletion technology, RiboFree Universal Depletion, that is compatible with total RNA from any organism. Therefore, this Total RNA-Seq library kit provides a versatile and valuable tool for whole transcriptome analysis in a myriad of scientific applications. Depletion of rRNAs (which can comprise as much as 90% of a total RNA sample) is completely integrated into the library preparation workflow of this kit. RiboFree Universal Depletion uses the input RNA as templates to drive the enzymatic removal of the reverse transcribed cDNA from the overly abundant ribosomal RNA sequences, thus eliminating the need to select, design or add organism-specific probes. RiboFree Universal Depletion is validated across biological kingdom and phyla, including human, rodent, avian, plant, and various prokaryotes, as well as RNA from a wide range of sample types including cells, snap-frozen tissues, FFPE tissues, and whole blood. It’s truly One for All: Universal rRNA Depletion for Any Organism. As the simplest library preparation method for total RNA-Seq, Zymo-Seq RiboFree Total RNA Library Kit simultaneously ligates both partial P7 and P5 adapters to the first-strand cDNAs in one step without adding extra artificial sequences; also, reagents in most sections of the protocol are in premixed formats ready for use. Such convenience greatly reduces hands-on processing. The automation-friendly protocol allows RNA to NGS libraries in as little as 4 hours. Everything needed to make sequencing-ready libraries, such as unique dual indexing primers and SPRI beads, are included in the kit. It’s RNA-Seq made simple.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch4\u003eHighlights\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003e    Universal Depletion: Novel probe-free technology depletes rRNA from any organism.\u003c\/li\u003e\n\u003cli\u003e    Simplest Library Prep: Simultaneous ligation of both adapters reduces hands-on processing.\u003c\/li\u003e\n\u003cli\u003e    Automation Friendly: Streamlined protocol for increased scalability.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTable 1. Zymo-Seq RiboFree Total RNA Library Kit (Cat. No. R3000\/R3003) update summary\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable border=\"1\" cellpadding=\"0\" cellspacing=\"1\" width=\"600\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e \u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eCurrent Version\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eUpdated Version\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eInstruction Manual Versions\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003ev1.3.0\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003ev2.0.0\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eMinimum Total RNA Input\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003e100 ng\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003e10 ng\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eDepletion Reaction Duration*\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003e100 ng input – 4 hours\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003e· 100 ng input – 1 hour\u003cbr\u003e· 10 ng input – 4 hours\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eHands-on Time\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003e4 rounds of bead cleanup\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003e3 rounds of bead cleanup\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cem\u003e* May vary depending on the quantity, quality, and source of the input RNA.\u003c\/em\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable border=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eEquipment Needed (user provided)\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eThermal cycler with heated lid, magnetic stand for 0.2 mL PCR tubes, microcentrifuge for 0.2 mL PCR tubes and 1.5 mL microcentrifuge tubes, and a benchtop vortex mixer.\u003cbr\u003e\u003cbr\u003eA complimentary magnet stand is available at online checkout for direct U.S. customers of R3000 and R3003.\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eInput Quality\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eRNA should be free of DNA contamination and enzymatic inhibitors, with A260\/A280 and A260\/A230 ≥ 1.8. RNA with lower purity ratios (A260\/A280 and A260\/A230) should be treated with DNase I and purified with the RNA Clean \u0026amp; Concentrator™ (Cat. No. R1013) prior to processing. RNA should be suspended in water, TE, or a low-salt buffer.\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cstrong\u003eFor optimal results, please use intact RNA (RNA Integrity Number or RIN ≥ 8.0) whenever possible.\u003c\/strong\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cstrong\u003eFor degraded RNA input, see Appendix E of the protocol for additional considerations and recommended modification.\u003c\/strong\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eLibrary Storage\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eLibraries eluted in DNA Elution Buffer (provided) may be stored at ≤ 4°C overnight or ≤ -20°C for long-term storage.\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eProcessing Time\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eAs little as 4 hours\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eRNA Input\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003e10 – 250 ng of total RNA.\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cstrong\u003eFor optimal results, please use the recommended 10-250 ng input. Do not use more than 250 ng.\u003c\/strong\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cstrong\u003eIf an input below 10 ng is necessary, see Appendix D of the protocol for additional considerations and recommended modifications.\u003c\/strong\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eSample Input Material\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eRNA from any species\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eSequencing Platform Compatibility\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eLibraries are compatible with all Illumina\u003csup\u003e®\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003esequencing platforms except HiSeq\u003csup\u003e®\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003eX.\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eIllumina originally limits the applications on HiSeq X exclusively for whole-genome libraries. Please confirm with the sequencing service provider for acceptability and additional details if expecting to sequence Zymo-Seq RiboFree Total RNA libraries on HiSeq X series sequencers.\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eSupplemental Info\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cstrong\u003eAny Organism. One rRNA Depletion Solution.\u003c\/strong\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cstrong\u003eEvaluating Quality of Input RNA for NGS Library Preparation\u003c\/strong\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan class=\"highlight\"\u003e\u003ca href=\"https:\/\/files.zymoresearch.com\/protocols\/r3000_zymo-seq_ribofree_total_rna_library_kit.pdf\" target=\"_blank\" title=\"Zymo-Seq RiboFree Total RNA Library Kit Protocol\" rel=\"noopener\"\u003eProtocol\u003c\/a\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/files.zymoresearch.com\/datasheets\/ds2701-06_zymo-seq_ribofree_total_rna_libary_kit_data_sheet.pdf\" target=\"_blank\" title=\"Zymo-Seq RiboFree Total RNA Library Kit Datasheet\" rel=\"noopener\"\u003e\u003cspan\u003e\u003cspan class=\"highlight\"\u003eDatasheet \u003c\/span\u003e\u003c\/span\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/files.zymoresearch.com\/sds\/sdr3000_-_zymo-seq_ribofree_total_rna_library_kit_(us).pdf\" target=\"_blank\" title=\"Zymo-Seq RiboFree Total RNA Library Kit SDS - R3000\" rel=\"noopener\"\u003e\u003cspan\u003e\u003cspan class=\"highlight\"\u003eSDS (MSDS)\u003c\/span\u003e\u003c\/span\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"Zymo Research","offers":[{"title":"Pack of 12 Preps","offer_id":44880692871322,"sku":"R3000","price":1412.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0593\/5025\/3722\/files\/R3000Zymo-SeqRiboFreeTotalRNALibraryKit_600x_bc3c920d-736f-41a3-a712-7c2817b335f5.webp?v=1715786351","url":"https:\/\/animal.ivfstore.com\/es\/products\/zymo-seq-ribofree-total-rna-library-kit","provider":"Animal IVF Store","version":"1.0","type":"link"}