{"product_id":"zymo-seq-cell-free-dna-wgbs-library-kit-96-prep","title":"Zymo-Seq Cell Free DNA WGBS Library Kit (96 prep)","description":"\u003ch2 id=\"ctl00_ctl00_ctl00_BodyContent_BodyContent_BodyContent_ctl00_ptPageTitle_h1Heading\" class=\"heading heading--1\" data-source=\"u-miscellaneous-pagetitle\"\u003e\u003cspan id=\"ctl00_ctl00_ctl00_BodyContent_BodyContent_BodyContent_ctl00_ptPageTitle_spanItemProp\"\u003e\u003cspan class=\"highlight\"\u003e\u003cspan class=\"highlight__text\"\u003eZymo\u003c\/span\u003e-Seq Cell Free DNA WGBS Library\u003cspan\u003e \u003c\/span\u003e\u003cspan class=\"highlight__text\"\u003eKit\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003e\u003cspan class=\"highlight\"\u003e\u003cspan\u003e\u003cmeta charset=\"utf-8\"\u003e \u003cspan class=\"highlight__text\"\u003eZymo\u003c\/span\u003e-Seq Cell Free DNA WGBS Library \u003cspan class=\"highlight__text\"\u003eKit\u003c\/span\u003e provides an optimized and reliable workflow for whole genome bisulfite sequencing (WGBS) library preparation from cell-free DNA (cfDNA). This all-inclusive kit features a straightforward procedure capable of preparing high-quality methyl-seq libraries from as little as 5 ng of cfDNA. The process is completed in three basic steps: bisulfite conversion, direct adapter ligation, and index PCR amplification. The initial bisulfite treatment is gentle on cfDNA yet effective in converting any unmodified cytosines into uracil. Next, the innovative splinted adapters capture and directly ligate the Illumina-compatible adapters onto any size DNA fragment, allowing for sequencing of nicked, damaged, and short DNA fragments that may have otherwise been discarded with traditional library prep methods. The direct ligation of the adapters also eliminates the need for second strand synthesis, end repair, and dA tailing steps, thus reducing bias by preserving the integrity of any native methylation that is present on the fragment termini. Finally, the adapter-ligated cfDNA is indexed and amplified via PCR with Zymo-Seq UDIs, generating libraries that are ready for sequencing on any Illumina instrument.\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch4\u003e\u003cspan\u003e\u003cspan class=\"highlight\"\u003e\u003cspan\u003eSpecifics\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/h4\u003e\n\u003ctable cellpadding=\"0\" border=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eBarcode Sequences\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003ePlease refer to the Documents Section.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eBisulfite Conversion\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u0026gt;99.5% of non-methylated cytosine residues are converted to uracil; \u0026gt;99.5% protection of methylated cytosines.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eEquipment Required\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003eThermal cycler(s) with temperature adjustable lids, microcentrifuge, magnetic stand.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eHands-On Time\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e~2 hours\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eInput Quality\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003eFor optimal results, use at least minimum input of purified cfDNA with no RNA or gDNA contamination. cfDNA can be concentrated using the\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/www.zymoresearch.com\/products\/dna-clean-concentrator-5\"\u003eDNA Clean \u0026amp; Concentrator (D4013)\u003c\/a\u003e\u003cspan\u003e \u003c\/span\u003eprior to processing. Input cfDNA can be suspended in water, DNA Elution Buffer, or TE buffer.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eLibrary Storage\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003eLibraries eluted in DNA Elution Buffer (provided) may be stored at ≤ 4°C overnight or ≤ -20°C for long-term storage.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eMaximum Input\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e10 ng\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eMinimum Input\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e5 ng\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eSample Input Material\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003ePurified cell-free DNA (cfDNA)\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eSequencing Compatibility\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003eLibraries are compatible with all Illumina sequencing platforms. Recommended: NextSeq, NovaSeq.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eTotal Processing Time\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e~6 hours\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003ca rel=\"noopener\" title=\"Zymo-Seq Cell Free DNA WGBS Library Kit Protocol Sheet\" href=\"https:\/\/files.zymoresearch.com\/protocols\/_d5462_d5463_zymo-seq_cell_free_dna_wgbs_library_kit.pdf\" target=\"_blank\"\u003e\u003cspan\u003e\u003cspan class=\"highlight\"\u003eProtocol \u003c\/span\u003e\u003c\/span\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca title=\"Zymo-Seq Cell Free DNA WGBS Library Kit FFPE Recommendations\" href=\"https:\/\/files.zymoresearch.com\/documents\/zymoseq_cell_free_dna_wgbs_library_kit__ffpe_dna_protocol_recommendations.pdf\"\u003eFFPE Recommendations\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca rel=\"noopener\" title=\"Zymo-Seq Cell Free DNA WGBS Library Kit Datasheet\" href=\"https:\/\/files.zymoresearch.com\/datasheets\/_ds2707_zymo-seq_cell_free_dna_wgbs_library_kit.pdf\" target=\"_blank\"\u003e\u003cspan\u003e\u003cspan class=\"highlight\"\u003eDatasheet \u003c\/span\u003e\u003c\/span\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan class=\"highlight\"\u003eSDS (MSDS): \u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca rel=\"noopener\" title=\"Zymo-Seq Cell Free DNA WGBS Library Kit (24 Preps) SDS\" href=\"https:\/\/files.zymoresearch.com\/sds\/_sdd5462_zymo-seq_cell_free_dna_wgbs_library_kit.pdf\" target=\"_blank\"\u003e\u003cspan\u003e\u003cspan class=\"highlight\"\u003eD5462\u003c\/span\u003e\u003c\/span\u003e\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca rel=\"noopener\" title=\"Zymo-Seq Cell Free DNA WGBS Library Kit (96 Preps) SDS\" href=\"https:\/\/files.zymoresearch.com\/sds\/_sdd5463_zymo-seq_cell_free_dna_wgbs_library_kit.pdf\" target=\"_blank\"\u003e\u003cspan\u003e\u003cspan class=\"highlight\"\u003eD5463\u003c\/span\u003e\u003c\/span\u003e\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Zymo Research","offers":[{"title":"1 EA \/ 24 Prep","offer_id":44880715579546,"sku":"D5462","price":1718.0,"currency_code":"USD","in_stock":false},{"title":"1 EA \/ 96 Prep","offer_id":44880715612314,"sku":"D5463","price":6590.0,"currency_code":"USD","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0593\/5025\/3722\/files\/D5462_Zymo-Seq-Cell-Free-DNA-WGBS-Library-Kit_600x_17529a9c-fe40-4950-aed9-ea1581f55b65.webp?v=1715786720","url":"https:\/\/animal.ivfstore.com\/es\/products\/zymo-seq-cell-free-dna-wgbs-library-kit-96-prep","provider":"Animal IVF Store","version":"1.0","type":"link"}