{"product_id":"zymo-environ-water-rna-kit","title":"Zymo Environ Water RNA Kit","description":"\u003ch2 id=\"ctl00_ctl00_ctl00_BodyContent_BodyContent_BodyContent_ctl00_ptPageTitle_h1Heading\" class=\"heading heading--1\" data-source=\"u-miscellaneous-pagetitle\"\u003e\u003cspan id=\"ctl00_ctl00_ctl00_BodyContent_BodyContent_BodyContent_ctl00_ptPageTitle_spanItemProp\"\u003e\u003cspan class=\"highlight\"\u003e\u003cspan class=\"highlight__text\"\u003eZymo\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003eEnviron Water RNA\u003cspan\u003e \u003c\/span\u003e\u003cspan class=\"highlight__text\"\u003eKit\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003eEnrich total RNA from large volumes (≤ 5 ml) and transform into small sample pellets for easy processing\u003c\/p\u003e\n\u003cp\u003eInactivates infectious agents and pathogens (viruses, bacteria, fungi, and parasites) for safe handling using DNA\/RNA Shield\u003c\/p\u003e\n\u003cp\u003eRemove PCR inhibitors in one spin and elute concentrated RNA in ≥6 µl. Ready for any downstream application including RT-qPCR, RTddPCR, NGS\u003c\/p\u003e\n\u003cp\u003e\u003cspan class=\"highlight\"\u003eThe\u003cspan\u003e \u003c\/span\u003e\u003cspan class=\"highlight__text\"\u003eZymo\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003eEnviron Water RNA\u003cspan\u003e \u003c\/span\u003e\u003cspan class=\"highlight__text\"\u003eKit\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003esimplifies COVID-19 wastewater surveillance by increasing viral enrichment, inactivating pathogens, and improving limits of detection. The use of Water Concentrating Buffer (WCB) promotes the pelleting of viral capsids and protein-associated RNA, increasing SARS-CoV-2 RNA yields by more than 8-fold. DNA\/RNA Shield allows for safe handling as it has been validated for inactivation of pathogens including SARS-CoV-2. Finally, the workflow yields highly concentrated, PCR inhibitor-free RNA ready for downstream applications including RT-qPCR, RT-ddPCR, and NGS.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch4\u003e\u003cspan class=\"highlight\"\u003eSpecifics \u003c\/span\u003e\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cspan class=\"highlight\"\u003e\u003cmeta charset=\"utf-8\"\u003e \u003cspan\u003eEquipment Needed - Microcentrifuge, vortex, large 50 mL conical tube centrifuge (optional), cell disrupter\/pulverizer (optional)\u003c\/span\u003e\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan class=\"highlight\"\u003e\u003cspan\u003eFormat - Bead beating, spin column\u003c\/span\u003e\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan class=\"highlight\"\u003e\u003cspan\u003eRNA Purity - High quality RNA (A260\/A280 \u0026gt; 1.8, A260\/A230 \u0026gt; 1.8) suitable for all downstream RNA-based manipulations\u003c\/span\u003e\u003cbr\u003e\u003cspan\u003eRNA Storage - RNA is eluted with RNase-free water and can be stored at ≤-70°C. The addition of RNase inhibitors is highly recommended for prolonged storage\u003c\/span\u003e\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan class=\"highlight\"\u003e\u003cspan\u003eSample Inactivation - DNA\/RNA Shield™ lyses cells, inactivates nucleases and infectious agents, and is ideal for safe sample storage and transport at ambient temperatures\u003c\/span\u003e\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan class=\"highlight\"\u003e\u003cspan\u003eSample Input - Water, wastewater, sludge, sewage, biofilms, etc.\u003c\/span\u003e\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan class=\"highlight\"\u003e\u003cspan\u003eSample Size - Up to 5 mL\u003c\/span\u003e\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan class=\"highlight\"\u003e\u003cspan\u003eYield - Up to 10 µg RNA can eluted into ≥ 6 µL RNase-free water allowing for a highly concentrated sample\u003c\/span\u003e\u003c\/span\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/files.zymoresearch.com\/protocols\/r2042-zymo_environ_water_rna_kit.pdf\" title=\"Zymo Environ Water RNA Kit Protocol\" rel=\"noopener\" target=\"_blank\"\u003e\u003cspan class=\"highlight\"\u003e\u003cspan\u003eProtocol \u003c\/span\u003e\u003c\/span\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan class=\"highlight\"\u003e\u003ca href=\"https:\/\/files.zymoresearch.com\/datasheets\/ds1310-zymo_environ_water_rna_kit.pdf\" title=\"Zymo Environ Water RNA Kit Datasheet\" rel=\"noopener\" target=\"_blank\"\u003eData Sheet\u003c\/a\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan class=\"highlight\"\u003e\u003ca href=\"https:\/\/files.zymoresearch.com\/sds\/sdr2042-zymo_environ_water_rna_kit-us.pdf\" title=\"Zymo Environ Water RNA Kit SDS US\" rel=\"noopener\" target=\"_blank\"\u003eSDS (MSDS)\u003c\/a\u003e \u003c\/span\u003e\u003c\/p\u003e","brand":"Zymo Research","offers":[{"title":"1 EA","offer_id":44877948944538,"sku":"","price":585.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0593\/5025\/3722\/files\/r2042-zymo_environ_water_rna_kit_5760x5760_74544de4-befb-40b6-a04d-32d7ba6924cd.webp?v=1715774912","url":"https:\/\/animal.ivfstore.com\/es\/products\/zymo-environ-water-rna-kit","provider":"Animal IVF Store","version":"1.0","type":"link"}