{"product_id":"puresperm-40-80-90","title":"PureSperm 40\/80\/90","description":"\u003ch2\u003ePureSperm 40\/80\/90 Gradient\u003c\/h2\u003e\n\u003cp\u003e\u003cb\u003eReady-diluted to save gradient preparation time.\u003c\/b\u003e\u003c\/p\u003e\n\u003cp\u003eAll the advantages of a \u003cb\u003e\u003ci\u003ePureSperm 100\u003c\/i\u003e\u003c\/b\u003e gradient, plus ready-diluted to save preparation time. \u003cb\u003e\u003ci\u003ePureSperm 40,\u003c\/i\u003e\u003c\/b\u003e \u003cb\u003e\u003ci\u003ePureSperm 80\u003c\/i\u003e\u003c\/b\u003e in both \u003cem\u003e100mL \u0026amp; 20mL\u003c\/em\u003e and \u003cb\u003e\u003ci\u003ePureSperm 90 \u003c\/i\u003e\u003c\/b\u003ein \u003cem\u003e100mL\u003c\/em\u003e, are formulated to minimize sudden pH and osmolarity changes during the transition of sperm from the semen sample to the fertilization medium. This helps to avoid premature hyperactivation and improves fertilization potential.\u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cb\u003e\u003ci\u003ePureSperm 40,\u003c\/i\u003e\u003c\/b\u003e \u003cb\u003e\u003ci\u003ePureSperm 80\u003c\/i\u003e\u003c\/b\u003e and \u003cb\u003e\u003ci\u003ePureSperm 90\u003c\/i\u003e\u003c\/b\u003e are formulated to minimize sudden pH and osmolarity changes during the transition of sperm from a semen sample to the fertilization medium. This helps to avoid premature hyperactivation and improves fertilization potential.\u003c\/li\u003e\n\u003cli\u003e\n\u003cb\u003e\u003ci\u003ePureSperm 40,\u003c\/i\u003e\u003c\/b\u003e \u003cb\u003e\u003ci\u003ePureSperm 80\u003c\/i\u003e\u003c\/b\u003e and \u003cb\u003e\u003ci\u003ePureSperm 90\u003c\/i\u003e\u003c\/b\u003e maintain pH between 7.4 – 7.8 at room temperature and at 37°C, providing suitable conditions for good sperm motility, survival time, and fertilization potential.\u003c\/li\u003e\n\u003cli\u003eThe preparation contains glucose as a usable energy substrate for normal cell metabolism and sperm function.\u003c\/li\u003e\n\u003cli\u003eThe gradient enables motile sperm to be separated from extraneous cells and seminal plasma. In addition, damage to sperm DNA and structural molecules caused by reactive oxygen species (ROS) is minimized by the effective removal of immature sperm and lymphocytes. Bacteria, epithelial cells, and cell debris are also separated from the motile sperm, which can be difficult to achieve in polysucrose-based gradient preparations.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eComponents\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eSilane-coated silica\u003c\/li\u003e\n\u003cli\u003ePotassium chloride\u003c\/li\u003e\n\u003cli\u003eSodium chloride\u003c\/li\u003e\n\u003cli\u003ePurified water\u003c\/li\u003e\n\u003cli\u003eHEPES\u003c\/li\u003e\n\u003cli\u003eEDTA\u003c\/li\u003e\n\u003cli\u003eGlucose\u003c\/li\u003e\n\u003cli\u003eSodium pyruvate\u003c\/li\u003e\n\u003cli\u003eCalcium chloride\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003ePerformance Characteristics\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003epH: 7.4 – 7.8.\u003c\/li\u003e\n\u003cli\u003eOsmolality (mOsm\/kg H2O) : 300 – 310.\u003c\/li\u003e\n\u003cli\u003eEndotoxin transfer during treatment : \u0026lt;1.0 EU\/mL.\u003c\/li\u003e\n\u003cli\u003eSperm survival 18 hours after density gradient separation: \u0026gt;70%.\u003c\/li\u003e\n\u003cli\u003eContents are tested by human sperm survival.\u003c\/li\u003e\n\u003cli\u003eBottles and stoppers are M.E.A. tested.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eStorage and Stability\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eStore unopened bottles at 2 to 27ºC and avoid temperatures above or below these values. Under these conditions \u003cb\u003e\u003ci\u003ePureSperm 40,\u003c\/i\u003e\u003c\/b\u003e \u003cb\u003e\u003ci\u003ePureSperm 80\u003c\/i\u003e\u003c\/b\u003e and \u003cb\u003e\u003ci\u003ePureSperm 90\u003c\/i\u003e\u003c\/b\u003e have a shelf-life of 24 months. The expiry date is shown on both bottles and cartons.\u003c\/li\u003e\n\u003cli\u003eOpen and close bottles under aseptic conditions. After opening, store at 2 to 8ºC when not in use. Shelf-life on the product label applies when the product is stored according to manufacturer’s recommendations.\u003c\/li\u003e\n\u003cli\u003eNo antibiotics, unstable additives or preservatives have been added by the manufacturer to \u003cb\u003e\u003ci\u003ePureSperm 40,\u003c\/i\u003e\u003c\/b\u003e \u003cb\u003e\u003ci\u003ePureSperm 80\u003c\/i\u003e\u003c\/b\u003e and \u003cb\u003e\u003ci\u003ePureSperm 90\u003c\/i\u003e\u003c\/b\u003e.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003ePrecautions and Warnings\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eWhen retrieving the sperm pellet, follow the instructions given in the pack insert to avoid inadvertent contamination.\u003c\/li\u003e\n\u003cli\u003eUse aseptic procedures at all times.\u003c\/li\u003e\n\u003cli\u003eIf available, use sealed centrifuge buckets during centrifugation to avoid creation of aerosols.\u003c\/li\u003e\n\u003cli\u003eClean accidental spills using a dampened cloth or paper.\u003cb\u003e\u003ci\u003ePureSperm 40,\u003c\/i\u003e\u003c\/b\u003e \u003cb\u003e\u003ci\u003ePureSperm 80\u003c\/i\u003e\u003c\/b\u003e and \u003cb\u003e\u003ci\u003ePureSperm 90\u003c\/i\u003e\u003c\/b\u003e cause floors and benches to be extremely slippery.\u003c\/li\u003e\n\u003cli\u003e\n\u003cb\u003e\u003ci\u003ePureSperm 40,\u003c\/i\u003e\u003c\/b\u003e \u003cb\u003e\u003ci\u003ePureSperm 80\u003c\/i\u003e\u003c\/b\u003e and \u003cb\u003e\u003ci\u003ePureSperm 90\u003c\/i\u003e\u003c\/b\u003e do not represent any kind of fire or combustion hazard. A material safety data sheet is available from the distributor or manufacturer(see nidacon.com).\u003c\/li\u003e\n\u003cli\u003eDo not use any solution which shows evidence of bacterial contamination.\u003c\/li\u003e\n\u003cli\u003eDo not use contents if tamper-evident seal is broken.\u003c\/li\u003e\n\u003cli\u003eFederal Law (USA) restricts this device to sale by or on the order of a physician.\u003c\/li\u003e\n\u003cli\u003ePlease check for regulatory compliance governing the use of ART products in your country.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eRecommendations for Viscous Samples\u003c\/h3\u003e\n\u003cp\u003eIt can be extremely difficult to obtain a high yield of motile sperm from highly viscous, semen samples. Nidacon has developed a method that can be helpful in solving this problem in the lab. You simply add PureSperm Buffer to the ejaculate, incubate for 15-30 minutes at 37°C and the sample is ready to use with reduced viscosity. Importantly, this will give you a much higher yield of motile sperm.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eProtocol:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eFor sperm preparation from a viscous semen sample:\u003cbr\u003e1. Bring all solutions to room temperature.\u003cbr\u003e2. Measure the volume of the semen sample.\u003cbr\u003e3. Dilute 1+2 , 1 part PureSperm Buffer and 2 parts semen sample\u003cbr\u003e(e.g. 0.5 ml PureSperm Buffer + 1.0 ml semen sample).\u003cbr\u003e4. Incubate at 37°C for 15-30 minutes.\u003cbr\u003e5. Mix using a pipette.\u003cbr\u003e6. Ready for sperm preparation on a density gradient.\u003c\/p\u003e\n\u003ch3\u003eDocuments\u003c\/h3\u003e\n\u003cp\u003eSDS – \u003ca href=\"https:\/\/www.nidacon.com\/wp-content\/uploads\/2018\/06\/PS4080DDA10-2.pdf\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eView PDF\u003cbr\u003e\u003c\/a\u003eProduct insert (latest version) – \u003ca href=\"https:\/\/www.nidacon.com\/wp-content\/uploads\/2017\/05\/insert-ps4080.pdf\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eView PDF 40\/80\u003c\/a\u003e or \u003ca href=\"https:\/\/www.nidacon.com\/wp-content\/uploads\/2017\/05\/insert-ps90.pdf\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eView PDF 90\u003cbr\u003e\u003c\/a\u003eLeaflet – \u003ca href=\"https:\/\/www.nidacon.com\/wp-content\/uploads\/2017\/05\/leaflet-ps408090.pdf\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eView PDF\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ciframe width=\"560\" height=\"315\" src=\"https:\/\/www.youtube.com\/embed\/AB6LMp8zlS8?rel=0\" frameborder=\"0\" allow=\"autoplay; encrypted-media\" allowfullscreen=\"\"\u003e\u003c\/iframe\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSpecifications:\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody style=\"font-size: 12px;\"\u003e\n\u003ctr\u003e\n\u003ctd\u003eMfr. No. \u003cspan\u003ePSK-020\u003c\/span\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003ePureSperm 40\/80 \/ 2 × 20 mL\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cspan\u003eMfr. No.  PS40-100 \u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003ePureSperm 40 \/ 100 mL\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cspan\u003eMfr. No.  PS80-100\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003ePureSperm 80 \/ 100 mL\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cspan\u003eMfr. No.  PS90-100\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003ePureSperm 90 \/ 100 mL (Not available in Europe)\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e","brand":"Nidacon","offers":[{"title":"1 EA \/ PureSperm 40\/80 \/ 2 × 20 mL","offer_id":44192982892698,"sku":"PSK-020","price":126.0,"currency_code":"USD","in_stock":true},{"title":"1 EA \/ PureSperm 40 \/ 100 mL","offer_id":44192982925466,"sku":"PS40-100","price":175.0,"currency_code":"USD","in_stock":true},{"title":"1 EA \/ PureSperm 80 \/ 100 mL","offer_id":44192982958234,"sku":"PS80-100","price":212.0,"currency_code":"USD","in_stock":true},{"title":"1 EA \/ PureSperm 90 \/ 100 mL","offer_id":44192982991002,"sku":"PS90-100","price":212.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0593\/5025\/3722\/products\/NIDACON-PURESPERM-40_80_90.png?v=1701801229","url":"https:\/\/animal.ivfstore.com\/es\/products\/puresperm-40-80-90","provider":"Animal IVF Store","version":"1.0","type":"link"}