{"title":"Equipo","description":"","products":[{"product_id":"zymo-environ-water-rna-kit","title":"Zymo Environ Water RNA Kit","description":"\u003ch2 id=\"ctl00_ctl00_ctl00_BodyContent_BodyContent_BodyContent_ctl00_ptPageTitle_h1Heading\" class=\"heading heading--1\" data-source=\"u-miscellaneous-pagetitle\"\u003e\u003cspan id=\"ctl00_ctl00_ctl00_BodyContent_BodyContent_BodyContent_ctl00_ptPageTitle_spanItemProp\"\u003e\u003cspan class=\"highlight\"\u003e\u003cspan class=\"highlight__text\"\u003eZymo\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003eEnviron Water RNA\u003cspan\u003e \u003c\/span\u003e\u003cspan class=\"highlight__text\"\u003eKit\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003eEnrich total RNA from large volumes (≤ 5 ml) and transform into small sample pellets for easy processing\u003c\/p\u003e\n\u003cp\u003eInactivates infectious agents and pathogens (viruses, bacteria, fungi, and parasites) for safe handling using DNA\/RNA Shield\u003c\/p\u003e\n\u003cp\u003eRemove PCR inhibitors in one spin and elute concentrated RNA in ≥6 µl. Ready for any downstream application including RT-qPCR, RTddPCR, NGS\u003c\/p\u003e\n\u003cp\u003e\u003cspan class=\"highlight\"\u003eThe\u003cspan\u003e \u003c\/span\u003e\u003cspan class=\"highlight__text\"\u003eZymo\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003eEnviron Water RNA\u003cspan\u003e \u003c\/span\u003e\u003cspan class=\"highlight__text\"\u003eKit\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003esimplifies COVID-19 wastewater surveillance by increasing viral enrichment, inactivating pathogens, and improving limits of detection. The use of Water Concentrating Buffer (WCB) promotes the pelleting of viral capsids and protein-associated RNA, increasing SARS-CoV-2 RNA yields by more than 8-fold. DNA\/RNA Shield allows for safe handling as it has been validated for inactivation of pathogens including SARS-CoV-2. Finally, the workflow yields highly concentrated, PCR inhibitor-free RNA ready for downstream applications including RT-qPCR, RT-ddPCR, and NGS.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch4\u003e\u003cspan class=\"highlight\"\u003eSpecifics \u003c\/span\u003e\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cspan class=\"highlight\"\u003e\u003cmeta charset=\"utf-8\"\u003e \u003cspan\u003eEquipment Needed - Microcentrifuge, vortex, large 50 mL conical tube centrifuge (optional), cell disrupter\/pulverizer (optional)\u003c\/span\u003e\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan class=\"highlight\"\u003e\u003cspan\u003eFormat - Bead beating, spin column\u003c\/span\u003e\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan class=\"highlight\"\u003e\u003cspan\u003eRNA Purity - High quality RNA (A260\/A280 \u0026gt; 1.8, A260\/A230 \u0026gt; 1.8) suitable for all downstream RNA-based manipulations\u003c\/span\u003e\u003cbr\u003e\u003cspan\u003eRNA Storage - RNA is eluted with RNase-free water and can be stored at ≤-70°C. The addition of RNase inhibitors is highly recommended for prolonged storage\u003c\/span\u003e\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan class=\"highlight\"\u003e\u003cspan\u003eSample Inactivation - DNA\/RNA Shield™ lyses cells, inactivates nucleases and infectious agents, and is ideal for safe sample storage and transport at ambient temperatures\u003c\/span\u003e\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan class=\"highlight\"\u003e\u003cspan\u003eSample Input - Water, wastewater, sludge, sewage, biofilms, etc.\u003c\/span\u003e\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan class=\"highlight\"\u003e\u003cspan\u003eSample Size - Up to 5 mL\u003c\/span\u003e\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan class=\"highlight\"\u003e\u003cspan\u003eYield - Up to 10 µg RNA can eluted into ≥ 6 µL RNase-free water allowing for a highly concentrated sample\u003c\/span\u003e\u003c\/span\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/files.zymoresearch.com\/protocols\/r2042-zymo_environ_water_rna_kit.pdf\" title=\"Zymo Environ Water RNA Kit Protocol\" rel=\"noopener\" target=\"_blank\"\u003e\u003cspan class=\"highlight\"\u003e\u003cspan\u003eProtocol \u003c\/span\u003e\u003c\/span\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan class=\"highlight\"\u003e\u003ca href=\"https:\/\/files.zymoresearch.com\/datasheets\/ds1310-zymo_environ_water_rna_kit.pdf\" title=\"Zymo Environ Water RNA Kit Datasheet\" rel=\"noopener\" target=\"_blank\"\u003eData Sheet\u003c\/a\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan class=\"highlight\"\u003e\u003ca href=\"https:\/\/files.zymoresearch.com\/sds\/sdr2042-zymo_environ_water_rna_kit-us.pdf\" title=\"Zymo Environ Water RNA Kit SDS US\" rel=\"noopener\" target=\"_blank\"\u003eSDS (MSDS)\u003c\/a\u003e \u003c\/span\u003e\u003c\/p\u003e","brand":"Zymo Research","offers":[{"title":"1 EA","offer_id":44877948944538,"sku":"","price":585.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0593\/5025\/3722\/files\/r2042-zymo_environ_water_rna_kit_5760x5760_74544de4-befb-40b6-a04d-32d7ba6924cd.webp?v=1715774912"},{"product_id":"zymo-spin-chip-kit","title":"Zymo-Spin ChIP Kit","description":"\u003ch1 id=\"ctl00_ctl00_ctl00_BodyContent_BodyContent_BodyContent_ctl00_ptPageTitle_h1Heading\" class=\"heading heading--1\" data-source=\"u-miscellaneous-pagetitle\" data-search-term-container=\"\"\u003e\u003cspan id=\"ctl00_ctl00_ctl00_BodyContent_BodyContent_BodyContent_ctl00_ptPageTitle_spanItemProp\"\u003e\u003cspan class=\"highlight\"\u003e\u003cspan class=\"highlight__text\"\u003eZymo\u003c\/span\u003e-Spin ChIP\u003cspan\u003e \u003c\/span\u003e\u003cspan class=\"highlight__text\"\u003eKit\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/h1\u003e\n\u003cp\u003e\u003cspan class=\"highlight\"\u003eThe\u003cspan\u003e \u003c\/span\u003e\u003cspan class=\"highlight__text\"\u003eZymo\u003c\/span\u003e-Spin ChIP\u003cspan\u003e \u003c\/span\u003e\u003cspan class=\"highlight__text\"\u003eKit\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003efrom Zymo Research provides a streamlined ChIP procedure for investigating protein-DNA interactions that have been \"fixed\" in their natural state and can be used to effectively identify binding sites for transcription factors, co-factors, and other DNA regulatory proteins.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch4\u003eHighlights:\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eRobust immunoprecipitation and purification of DNA.\u003c\/li\u003e\n\u003cli\u003eUnique workflow features a micro-elution (≥6 µl) spin column for purification of ChIP DNA.\u003c\/li\u003e\n\u003cli\u003eHigh-quality ChIP DNA is ideal for ChIP-qPCR, ChIP-Seq, and other molecular applications.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cspan class=\"highlight\"\u003e\u003ca href=\"https:\/\/files.zymoresearch.com\/protocols\/_d5209_d5210_zymo-spin_chip_kit.pdf\" target=\"_blank\" title=\"Zymo-Spin ChIP Kit Protocol\" rel=\"noopener\"\u003eProtocol\u003c\/a\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/files.zymoresearch.com\/datasheets\/ds1507_zymo-spin_chip_data_sheet.pdf\" target=\"_blank\" title=\"Zymo-Spin ChIP Kit Datasheet\" rel=\"noopener\"\u003e\u003cspan\u003e\u003cspan class=\"highlight\"\u003eDatasheet \u003c\/span\u003e\u003c\/span\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cspan class=\"highlight\"\u003eSDS (MSDS)\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/files.zymoresearch.com\/sds\/_d5209_zymo-spin_chip_kit.pdf\" target=\"_blank\" title=\"Zymo-Spin ChIP Kit SDS - D5209\" rel=\"noopener\"\u003e\u003cspan\u003e\u003cspan class=\"highlight\"\u003eD5209\u003c\/span\u003e\u003c\/span\u003e\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/files.zymoresearch.com\/sds\/_d5210_zymo-spin_chip_kit.pdf\" target=\"_blank\" title=\"Zymo-Spin ChIP Kit SDS - D5210\" rel=\"noopener\"\u003e\u003cspan\u003e\u003cspan class=\"highlight\"\u003eD5210\u003c\/span\u003e\u003c\/span\u003e\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Zymo Research","offers":[{"title":"1 EA \/ Zymo-Spin ChIP Kit (10 Preps.)","offer_id":44880500326554,"sku":"D5209","price":286.4,"currency_code":"USD","in_stock":true},{"title":"1 EA \/ Zymo-Spin ChIP Kit (25 Preps.)","offer_id":44880500359322,"sku":"D5210","price":587.6,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0593\/5025\/3722\/files\/D5209_Zymo-Spin-ChIP-Kit_600x_9db04e77-b2bd-4df4-a437-e4c7e6ae1c7c.webp?v=1715776002"},{"product_id":"zymo-seq-wgbs-library-kit","title":"Zymo-Seq WGBS Library Kit","description":"\u003ch1 id=\"ctl00_ctl00_ctl00_BodyContent_BodyContent_BodyContent_ctl00_ptPageTitle_h1Heading\" class=\"heading heading--1\" data-source=\"u-miscellaneous-pagetitle\" data-search-term-container=\"\"\u003e\u003cspan id=\"ctl00_ctl00_ctl00_BodyContent_BodyContent_BodyContent_ctl00_ptPageTitle_spanItemProp\"\u003e\u003cspan class=\"highlight\"\u003e\u003cspan class=\"highlight__text\"\u003eZymo\u003c\/span\u003e-Seq WGBS Library\u003cspan\u003e \u003c\/span\u003e\u003cspan class=\"highlight__text\"\u003eKit\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/h1\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cspan class=\"highlight\"\u003e\u003cspan class=\"highlight__text\"\u003eZymo\u003c\/span\u003e-Seq WGBS Library\u003cspan\u003e \u003c\/span\u003e\u003cspan class=\"highlight__text\"\u003eKit\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003eis the only kit available for whole genome bisulfite (WGBS) library preparation in a single tube. Zymo-Seq WGBS Library Kit incorporates tagmentation technology to eliminate the tedious fragmentation, enzymatic, and clean-up steps required by conventional, ligation-based library preparation methods. This streamlined workflow minimizes hands-on time, making it an ideal choice for higher throughput applications. To prepare Zymo-Seq WGBS libraries, intact genomic DNA is first bisulfite converted. Then, the following library preparation procedures are completed in a single tube: (1) second-strand synthesis, (2) adapterization via tagmentation, and (3) library amplification and indexing. After purification, libraries are ready for sequencing on Illumina instruments.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cbr data-mce-fragment=\"1\"\u003e\u003cspan class=\"highlight\"\u003eWGBS is the gold standard for DNA methylation studies as it provides base-level methylation quantification for all cytosines.\u003cspan\u003e \u003c\/span\u003e\u003cspan class=\"highlight__text\"\u003eZymo\u003c\/span\u003e-Seq WGBS Library\u003cspan\u003e \u003c\/span\u003e\u003cspan class=\"highlight__text\"\u003eKit\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003eis ideal for any species for whole-genome methylation calling of CG, CHG, and CHH sites. Over 90% of human and mouse CpG sites can be detected from Zymo-Seq libraries.\u003c\/span\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli class=\"text-secondary list-unstyled mb-3 pr-2\"\u003eWhole Genome Bisulfite (WGBS) library preparation in less than 4 hours and in one tube.\u003c\/li\u003e\n\u003cli class=\"text-secondary list-unstyled mb-3 pr-2\"\u003eReduced library preparation bias for accurate methylation calling\u003c\/li\u003e\n\u003cli class=\"text-secondary list-unstyled mb-3 pr-2\"\u003eReproducible genome coverage from any species (\u0026gt;90% of CpG sites detected in mammalian samples)\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003eDNA Input\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eFor optimal results, use 100 ng of high quality, intact genomic DNA as input. Protocol is compatible with inputs between 10 ng to 100 ng. DNA should be free of enzymatic inhibitors and RNA contamination. DNA can be resuspended in water, TE, or a low salt buffer.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eProcessing Time\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e~ 4 hours\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eRequired Equipment\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eMicrocentrifuge, thermal cycler with heated lid, magnetic separator, multichannel pipette (suggested)\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eRequired material not provided\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eIllumina DNA Prep Kit (Illumina, Cat. No. 20018704 or 20018705)\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSequencing Platform\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eLibraries are compatible with all Illumina sequencing platforms (MiSeq, MiniSeq, HiSeq, NextSeq, NovaSeq)\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/files.zymoresearch.com\/protocols\/d5465-zymo_seq_wgbs_library_kit.pdf\" target=\"_blank\" title=\"Zymo-Seq WGBS Library Kit Protocol\" rel=\"noopener\"\u003e\u003cspan\u003e\u003cspan class=\"highlight\"\u003eProtocol \u003c\/span\u003e\u003c\/span\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/files.zymoresearch.com\/datasheets\/ds2705-zymo_seq_wgbs_library_kit.pdf\" target=\"_blank\" title=\"Zymo-Seq WGBS Library Kit Datasheet\" rel=\"noopener\"\u003e\u003cspan\u003e\u003cspan class=\"highlight\"\u003eDatasheet \u003c\/span\u003e\u003c\/span\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/files.zymoresearch.com\/sds\/sdd5465-zymo_seq_wgbs_library_kit-us.pdf\" target=\"_blank\" title=\"Zymo-Seq WGBS Library Kit SDS\" rel=\"noopener\"\u003e\u003cspan\u003e\u003cspan class=\"highlight\"\u003eSDS (MSDS)\u003c\/span\u003e\u003c\/span\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"Zymo Research","offers":[{"title":"1 EA","offer_id":44879073607834,"sku":"D5465","price":1185.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0593\/5025\/3722\/files\/d5465-zymo_seq_wgbs_library_kit_600x_ddb91a3a-298a-44ae-954e-cf57730e7dc6.webp?v=1715781551"},{"product_id":"zymo-seq-rrbs-library-kit","title":"Zymo-Seq RRBS Library Kit","description":"\u003ch2 id=\"ctl00_ctl00_ctl00_BodyContent_BodyContent_BodyContent_ctl00_ptPageTitle_h1Heading\" class=\"heading heading--1\" data-source=\"u-miscellaneous-pagetitle\"\u003e\u003cspan id=\"ctl00_ctl00_ctl00_BodyContent_BodyContent_BodyContent_ctl00_ptPageTitle_spanItemProp\"\u003e\u003cspan class=\"highlight\"\u003e\u003cspan class=\"highlight__text\"\u003eZymo\u003c\/span\u003e-Seq RRBS Library\u003cspan\u003e \u003c\/span\u003e\u003cspan class=\"highlight__text\"\u003eKit\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003e\u003cmeta charset=\"utf-8\"\u003e \u003cspan class=\"highlight__text\"\u003eZymo\u003c\/span\u003e\u003cspan class=\"highlight\"\u003e-Seq RRBS Library \u003cspan class=\"highlight__text\"\u003eKit\u003c\/span\u003e is an all-in-one RRBS library prep kit that generates NGS libraries with a simplified workflow for the classic method Reduced Representation Bisulfite Sequencing (RRBS). This all-in-one RRBS library prep kit allows for DNA methylation profiling at single-nucleotide resolution in CpG-rich regions of the genome. This Zymo-Seq RRBS Library Kit is the ideal RRBS library prep kit to generate libraries that cover a significant percent (=70% if using human genomic DNA) of all CpG islands, promotors, and gene bodies while largely reduces the sequencing cost, making it ideal for high-throughput screening and comparison experiments.  Now includes Unique Dual Index (UDI) Primer Sets.\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch4\u003e\u003cspan\u003e\u003cspan class=\"highlight\"\u003eSpecifics\u003c\/span\u003e\u003c\/span\u003e\u003c\/h4\u003e\n\u003ctable class=\"table spec-table\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eEquipment Required\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003eMicrocentrifuge, thermocycler with a heated lid\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eInput\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e10 – 500 ng of genomic DNA\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSample Sources\u003c\/td\u003e\n\u003ctd\u003e\n\u003cspan\u003ePurified genomic DNA. The DNA should be free of enzymatic inhibitors and can be suspended in water, TE, or a low salt buffer. DNA with low 260\/280 or 260\/230 ratios should be purified prior to processing using the \u003c\/span\u003e\u003cstrong\u003eGenomic DNA Clean \u0026amp; Concentrator (Cat. No. D4010)\u003c\/strong\u003e\u003cspan\u003e. This protocol is not recommended for samples from species with low CpG density.\u003c\/span\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSequencing Platform Compatible\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003eLibraries are compatible with all Illumina sequencing platforms except HiSeq X Series.\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan class=\"highlight\"\u003e\u003ca href=\"https:\/\/files.zymoresearch.com\/protocols\/d5460_d5461_zymo-seq_rrbs_library_kit.pdf\" target=\"_blank\" title=\"Zymo-Seq RRBS Library Kit Protocol\" rel=\"noopener\"\u003eProtocol\u003c\/a\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/files.zymoresearch.com\/datasheets\/ds2702_zymo-seq_rrbs_library_kit.pdf\" target=\"_blank\" title=\"Zymo-Seq RRBS Library Kit Datasheet\" rel=\"noopener\"\u003e\u003cspan\u003e\u003cspan class=\"highlight\"\u003eDatasheet \u003c\/span\u003e\u003c\/span\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/files.zymoresearch.com\/sds\/d5460_d5461-ez_dna_methylation_rrbs_library_prep_kit.pdf\" target=\"_blank\" title=\"Zymo-Seq RRBS Library Kit SDS\" rel=\"noopener\"\u003e\u003cspan\u003e\u003cspan class=\"highlight\"\u003eSDS (MSDS)\u003c\/span\u003e\u003c\/span\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"Zymo Research","offers":[{"title":"Pack of 48 Preps","offer_id":44880487481498,"sku":"D5461","price":3210.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0593\/5025\/3722\/files\/d5460_ez_dna_methylation_rrbs_library_prep_kit_600x_031818c7-f06e-48d5-ad44-9a96c6c39c9d.webp?v=1715782403"},{"product_id":"zymo-rna-clean-and-concentrator-100-kit-25-preps","title":"Zymo RNA Clean and Concentrator - 100 Kit, 25 Preps","description":"\u003ch2 id=\"ctl00_ctl00_ctl00_BodyContent_BodyContent_BodyContent_ctl00_ptPageTitle_h1Heading\" class=\"heading heading--1\" data-source=\"u-miscellaneous-pagetitle\"\u003e\u003cspan id=\"ctl00_ctl00_ctl00_BodyContent_BodyContent_BodyContent_ctl00_ptPageTitle_spanItemProp\"\u003e\u003cspan class=\"highlight\"\u003e\u003cspan class=\"highlight__text\"\u003eZymo\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003eRNA Clean and Concentrator - 100\u003cspan\u003e \u003c\/span\u003e\u003cspan class=\"highlight__text\"\u003eKit\u003c\/span\u003e, 25 Preps\u003c\/span\u003e\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003e\u003cmeta charset=\"utf-8\"\u003eThe RNA Clean \u0026amp; Concentrator-100 provides a simple and reliable method for the rapid preparation of high-quality RNA. This simple procedure is based on the use of a unique single-buffer system and \u003cspan class=\"highlight__text\"\u003eZymo\u003c\/span\u003e\u003cspan class=\"highlight\"\u003e-Spin column technology. The procedure is easy: just add the binding buffer to your sample, adjust the conditions for binding by adding ethanol, wash, and elute the concentrated RNA. RNA 17 bases can be safely treated and recovered using this \u003cspan class=\"highlight__text\"\u003ekit\u003c\/span\u003e. The result is highly-concentrated, purified RNA that is suitable for subsequent RNA-based methods including RT-PCR, hybridization, etc.\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/files.zymoresearch.com\/protocols\/_r1019_rna_clean_concentrator-100.pdf\" target=\"_blank\" title=\"Zymo RNA Clean and Concentrator Protocol sheet\" rel=\"noopener\"\u003e\u003cspan\u003e\u003cspan class=\"highlight\"\u003eProtocol \u003c\/span\u003e\u003c\/span\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/files.zymoresearch.com\/datasheets\/ds1308-03_rna_clean_concentrator_datasheet.pdf\" title=\"Zymo RNA Clean and Concentrator Datasheet\"\u003e\u003cspan\u003e\u003cspan class=\"highlight\"\u003eDatasheet \u003c\/span\u003e\u003c\/span\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/files.zymoresearch.com\/sds\/_r1019_rna_clean_concentrator-100_25_preps.pdf\" title=\"Zymo RNA Clean and Concentrator SDS\"\u003e\u003cspan\u003e\u003cspan class=\"highlight\"\u003eSDS (MSDS)\u003c\/span\u003e\u003c\/span\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"Zymo Research","offers":[{"title":"1 EA","offer_id":44880560652442,"sku":"R1019","price":296.4,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0593\/5025\/3722\/files\/R1019_RNA-Clean-_-Concentrator-100_600x_cbe6a1fa-ee3e-4e80-b3df-005494ba4cbf.webp?v=1715783705"},{"product_id":"zymo-seq-ribofree-universal-cdna-kit","title":"Zymo-Seq RiboFree Universal cDNA Kit","description":"\u003ch1 id=\"ctl00_ctl00_ctl00_BodyContent_BodyContent_BodyContent_ctl00_ptPageTitle_h1Heading\" class=\"heading heading--1\" data-source=\"u-miscellaneous-pagetitle\" data-search-term-container=\"\"\u003e\u003cspan id=\"ctl00_ctl00_ctl00_BodyContent_BodyContent_BodyContent_ctl00_ptPageTitle_spanItemProp\"\u003e\u003cspan class=\"highlight\"\u003e\u003cspan class=\"highlight__text\"\u003eZymo\u003c\/span\u003e-Seq RiboFree Universal cDNA\u003cspan\u003e \u003c\/span\u003e\u003cspan class=\"highlight__text\"\u003eKit\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/h1\u003e\n\u003ch4\u003eDescription\u003c\/h4\u003e\n\u003cp\u003e\u003cspan class=\"highlight\"\u003eThe\u003cspan\u003e \u003c\/span\u003e\u003cspan class=\"highlight__text\"\u003eZymo\u003c\/span\u003e-Seq RiboFree Universal cDNA\u003cspan\u003e \u003c\/span\u003e\u003cspan class=\"highlight__text\"\u003eKit\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003eis the simplest kit for the preparation of NGS-grade first-strand cDNA with ribosomal RNA (rRNA) depleted. This simple cDNA kit features RiboFree Universal Depletion, a novel probe-free rRNA depletion technology that depletes rRNA from any organism. The RiboFree Universal Depletion is validated across biological kingdom and phyla, including mammals, plants, and various prokaryotes, and also RNA from a wide range of sample types including cells, snap-frozen tissues, and FFPE samples. The RiboFree single-stranded cDNA produced by the kit can be used for qPCR detection, RNA-Seq library prep, and any other downstream analysis. In contrast to poly(A) enrichment methods, the RiboFree Universal cDNA Kit produces single-stranded cDNA from nearly all RNA biotypes, including full-length mRNA, mRNA with degraded poly(A) tails, long-noncoding RNA (lncRNA), intronic RNA, nucleolar RNA, etc. The cost-effective Zymo-Seq RiboFree Universal cDNA Kit has been optimized to reduce hands-on time, with all the reagents such as SPRI beads included for researchers’ convenience.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch4\u003eHighlights\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eUniversal Depletion: Novel probe-free technology depletes rRNA from any organism.\u003c\/li\u003e\n\u003cli\u003e\u003cspan class=\"highlight\"\u003eSimplest\u003cspan\u003e \u003c\/span\u003e\u003cspan class=\"highlight__text\"\u003eKit\u003c\/span\u003e: Prepare NGS-grade RiboFree cDNA from total RNA in as little as 1.75 hours.\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003eAutomation Friendly: Streamlined protocol for increased scalability.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003eTable 2. Zymo-Seq RiboFree Universal cDNA Kit (Cat. No. R3001) update summary\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable border=\"1\" cellpadding=\"0\" cellspacing=\"1\" width=\"600\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e \u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eCurrent Version\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eUpdated Version\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eInstruction Manual Versions\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003ev1.1.0\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003ev2.0.0\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eTotal RNA Input Range\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e100 ng – 5 µg\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e10 ng – 1000 ng\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003eDepletion Reaction Duration*\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e100 ng input – 4 hours\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e· 100 ng input – 1 hour\u003cbr\u003e· 10 ng input – 4 hours\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cem\u003e* May vary depending on the quantity, quality, and source of the input RNA.\u003c\/em\u003e\u003c\/p\u003e\n\u003ctable border=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eEquipment Needed (user provided)\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003eThermal cycler with heated lid, magnetic stand for 0.2 mL PCR tubes, microcentrifuge for 0.2 mL PCR tubes and 1.5 mL microcentrifuge tubes, and a benchtop vortex mixer.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eSample Input Material\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003eRNA\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eRNA Input\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e10 – 1000 ng of total RNA.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eFor optimal results, please use the recommended 10-1000 ng input.\u003c\/li\u003e\n\u003cli\u003eIf an input below 10 ng is necessary, see\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003eAppendix B\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eof the kit protocol for additional considerations and recommended modifications.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eInput Quality\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003eRNA should be free of DNA contamination and enzymatic inhibitors, with A260\/A280 and A260\/A230\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003e≥ 1.8\u003c\/strong\u003e. RNA with lower purity ratios (A260\/A280 and A260\/A230) should be treated with DNase I and purified with the\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003eRNA Clean \u0026amp; Concentrator™ (Cat. No. R1013)\u003cspan\u003e \u003c\/span\u003e\u003c\/strong\u003eprior to processing. RNA should be suspended in water, TE, or a low-salt buffer.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eFor optimal results, please use intact RNA (RNA Integrity Number or RIN ≥ 8.0) whenever possible.\u003c\/li\u003e\n\u003cli\u003eFor degraded RNA input, see\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003eAppendix C\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eof the protocol for additional considerations and recommended modifications.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eProcessing Time\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003eAs little as 1.75 hours\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan class=\"highlight\"\u003e\u003ca href=\"https:\/\/files.zymoresearch.com\/protocols\/r3001_zymo-seq_ribofree_universal_cdna_kit.pdf\" target=\"_blank\" title=\"Zymo-Seq RiboFree Universal cDNA Kit Protocol\" rel=\"noopener\"\u003eProtocol\u003c\/a\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/files.zymoresearch.com\/datasheets\/ds2703_02_zymo-seq_ribofree_universal_cdna_kit_data_sheet.pdf\" target=\"_blank\" title=\"Zymo-Seq RiboFree Universal cDNA Kit Datasheet\" rel=\"noopener\"\u003e\u003cspan\u003e\u003cspan class=\"highlight\"\u003eDatasheet \u003c\/span\u003e\u003c\/span\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/files.zymoresearch.com\/sds\/sdr3001_-_zymo-seq_ribofree_universal_cdna_kit_(US).pdf\" title=\"Zymo-Seq RiboFree Universal cDNA Kit SDS - R3001\" rel=\"noopener\" target=\"_blank\"\u003e\u003cspan\u003e\u003cspan class=\"highlight\"\u003eSDS (MSDS)\u003c\/span\u003e\u003c\/span\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"Zymo Research","offers":[{"title":"Pack of 12 Preps","offer_id":44880577462426,"sku":"R3001","price":706.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0593\/5025\/3722\/files\/Zymo-SeqRiboFreeUniversalcDNAKit_Custom_600x_7b4480bd-e01a-40ba-be48-9a2ded77d9e3.webp?v=1715784603"},{"product_id":"zymo-seq-ribofree-total-rna-library-kit","title":"Zymo-Seq RiboFree Total RNA Library Kit","description":"\u003ch2 id=\"ctl00_ctl00_ctl00_BodyContent_BodyContent_BodyContent_ctl00_ptPageTitle_h1Heading\" class=\"heading heading--1\" data-source=\"u-miscellaneous-pagetitle\"\u003e\u003cspan id=\"ctl00_ctl00_ctl00_BodyContent_BodyContent_BodyContent_ctl00_ptPageTitle_spanItemProp\"\u003e\u003cspan class=\"highlight\"\u003e\u003cspan class=\"highlight__text\"\u003eZymo\u003c\/span\u003e-Seq RiboFree Total RNA Library\u003cspan\u003e \u003c\/span\u003e\u003cspan class=\"highlight__text\"\u003eKit\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/h2\u003e\n\u003ch4\u003eDescription\u003c\/h4\u003e\n\u003cp\u003e\u003cspan class=\"highlight\"\u003e\u003cspan class=\"highlight__text\"\u003eZymo\u003c\/span\u003e-Seq RiboFree Total RNA Library\u003cspan\u003e \u003c\/span\u003e\u003cspan class=\"highlight__text\"\u003eKit\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003eis the simplest RNA-Seq library prep kit available to make stranded, ribosomal RNA (rRNA) depleted, total RNA libraries. This all-inclusive RNA-Seq library prep kit features a novel probe-free rRNA depletion technology, RiboFree Universal Depletion, that is compatible with total RNA from any organism. Therefore, this Total RNA-Seq library kit provides a versatile and valuable tool for whole transcriptome analysis in a myriad of scientific applications. Depletion of rRNAs (which can comprise as much as 90% of a total RNA sample) is completely integrated into the library preparation workflow of this kit. RiboFree Universal Depletion uses the input RNA as templates to drive the enzymatic removal of the reverse transcribed cDNA from the overly abundant ribosomal RNA sequences, thus eliminating the need to select, design or add organism-specific probes. RiboFree Universal Depletion is validated across biological kingdom and phyla, including human, rodent, avian, plant, and various prokaryotes, as well as RNA from a wide range of sample types including cells, snap-frozen tissues, FFPE tissues, and whole blood. It’s truly One for All: Universal rRNA Depletion for Any Organism. As the simplest library preparation method for total RNA-Seq, Zymo-Seq RiboFree Total RNA Library Kit simultaneously ligates both partial P7 and P5 adapters to the first-strand cDNAs in one step without adding extra artificial sequences; also, reagents in most sections of the protocol are in premixed formats ready for use. Such convenience greatly reduces hands-on processing. The automation-friendly protocol allows RNA to NGS libraries in as little as 4 hours. Everything needed to make sequencing-ready libraries, such as unique dual indexing primers and SPRI beads, are included in the kit. It’s RNA-Seq made simple.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch4\u003eHighlights\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003e    Universal Depletion: Novel probe-free technology depletes rRNA from any organism.\u003c\/li\u003e\n\u003cli\u003e    Simplest Library Prep: Simultaneous ligation of both adapters reduces hands-on processing.\u003c\/li\u003e\n\u003cli\u003e    Automation Friendly: Streamlined protocol for increased scalability.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTable 1. Zymo-Seq RiboFree Total RNA Library Kit (Cat. No. R3000\/R3003) update summary\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable border=\"1\" cellpadding=\"0\" cellspacing=\"1\" width=\"600\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e \u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eCurrent Version\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eUpdated Version\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eInstruction Manual Versions\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003ev1.3.0\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003ev2.0.0\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eMinimum Total RNA Input\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003e100 ng\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003e10 ng\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eDepletion Reaction Duration*\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003e100 ng input – 4 hours\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003e· 100 ng input – 1 hour\u003cbr\u003e· 10 ng input – 4 hours\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eHands-on Time\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003e4 rounds of bead cleanup\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003e3 rounds of bead cleanup\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cem\u003e* May vary depending on the quantity, quality, and source of the input RNA.\u003c\/em\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable border=\"0\" cellpadding=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eEquipment Needed (user provided)\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eThermal cycler with heated lid, magnetic stand for 0.2 mL PCR tubes, microcentrifuge for 0.2 mL PCR tubes and 1.5 mL microcentrifuge tubes, and a benchtop vortex mixer.\u003cbr\u003e\u003cbr\u003eA complimentary magnet stand is available at online checkout for direct U.S. customers of R3000 and R3003.\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eInput Quality\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eRNA should be free of DNA contamination and enzymatic inhibitors, with A260\/A280 and A260\/A230 ≥ 1.8. RNA with lower purity ratios (A260\/A280 and A260\/A230) should be treated with DNase I and purified with the RNA Clean \u0026amp; Concentrator™ (Cat. No. R1013) prior to processing. RNA should be suspended in water, TE, or a low-salt buffer.\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cstrong\u003eFor optimal results, please use intact RNA (RNA Integrity Number or RIN ≥ 8.0) whenever possible.\u003c\/strong\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cstrong\u003eFor degraded RNA input, see Appendix E of the protocol for additional considerations and recommended modification.\u003c\/strong\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eLibrary Storage\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eLibraries eluted in DNA Elution Buffer (provided) may be stored at ≤ 4°C overnight or ≤ -20°C for long-term storage.\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eProcessing Time\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eAs little as 4 hours\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eRNA Input\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003e10 – 250 ng of total RNA.\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cstrong\u003eFor optimal results, please use the recommended 10-250 ng input. Do not use more than 250 ng.\u003c\/strong\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cstrong\u003eIf an input below 10 ng is necessary, see Appendix D of the protocol for additional considerations and recommended modifications.\u003c\/strong\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eSample Input Material\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eRNA from any species\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eSequencing Platform Compatibility\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eLibraries are compatible with all Illumina\u003csup\u003e®\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003esequencing platforms except HiSeq\u003csup\u003e®\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003eX.\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eIllumina originally limits the applications on HiSeq X exclusively for whole-genome libraries. Please confirm with the sequencing service provider for acceptability and additional details if expecting to sequence Zymo-Seq RiboFree Total RNA libraries on HiSeq X series sequencers.\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eSupplemental Info\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cstrong\u003eAny Organism. One rRNA Depletion Solution.\u003c\/strong\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cstrong\u003eEvaluating Quality of Input RNA for NGS Library Preparation\u003c\/strong\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan class=\"highlight\"\u003e\u003ca href=\"https:\/\/files.zymoresearch.com\/protocols\/r3000_zymo-seq_ribofree_total_rna_library_kit.pdf\" target=\"_blank\" title=\"Zymo-Seq RiboFree Total RNA Library Kit Protocol\" rel=\"noopener\"\u003eProtocol\u003c\/a\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/files.zymoresearch.com\/datasheets\/ds2701-06_zymo-seq_ribofree_total_rna_libary_kit_data_sheet.pdf\" target=\"_blank\" title=\"Zymo-Seq RiboFree Total RNA Library Kit Datasheet\" rel=\"noopener\"\u003e\u003cspan\u003e\u003cspan class=\"highlight\"\u003eDatasheet \u003c\/span\u003e\u003c\/span\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/files.zymoresearch.com\/sds\/sdr3000_-_zymo-seq_ribofree_total_rna_library_kit_(us).pdf\" target=\"_blank\" title=\"Zymo-Seq RiboFree Total RNA Library Kit SDS - R3000\" rel=\"noopener\"\u003e\u003cspan\u003e\u003cspan class=\"highlight\"\u003eSDS (MSDS)\u003c\/span\u003e\u003c\/span\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"Zymo Research","offers":[{"title":"Pack of 12 Preps","offer_id":44880692871322,"sku":"R3000","price":1412.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0593\/5025\/3722\/files\/R3000Zymo-SeqRiboFreeTotalRNALibraryKit_600x_bc3c920d-736f-41a3-a712-7c2817b335f5.webp?v=1715786351"},{"product_id":"zymo-seq-cell-free-dna-wgbs-library-kit-96-prep","title":"Zymo-Seq Cell Free DNA WGBS Library Kit (96 prep)","description":"\u003ch2 id=\"ctl00_ctl00_ctl00_BodyContent_BodyContent_BodyContent_ctl00_ptPageTitle_h1Heading\" class=\"heading heading--1\" data-source=\"u-miscellaneous-pagetitle\"\u003e\u003cspan id=\"ctl00_ctl00_ctl00_BodyContent_BodyContent_BodyContent_ctl00_ptPageTitle_spanItemProp\"\u003e\u003cspan class=\"highlight\"\u003e\u003cspan class=\"highlight__text\"\u003eZymo\u003c\/span\u003e-Seq Cell Free DNA WGBS Library\u003cspan\u003e \u003c\/span\u003e\u003cspan class=\"highlight__text\"\u003eKit\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003e\u003cspan class=\"highlight\"\u003e\u003cspan\u003e\u003cmeta charset=\"utf-8\"\u003e \u003cspan class=\"highlight__text\"\u003eZymo\u003c\/span\u003e-Seq Cell Free DNA WGBS Library \u003cspan class=\"highlight__text\"\u003eKit\u003c\/span\u003e provides an optimized and reliable workflow for whole genome bisulfite sequencing (WGBS) library preparation from cell-free DNA (cfDNA). This all-inclusive kit features a straightforward procedure capable of preparing high-quality methyl-seq libraries from as little as 5 ng of cfDNA. The process is completed in three basic steps: bisulfite conversion, direct adapter ligation, and index PCR amplification. The initial bisulfite treatment is gentle on cfDNA yet effective in converting any unmodified cytosines into uracil. Next, the innovative splinted adapters capture and directly ligate the Illumina-compatible adapters onto any size DNA fragment, allowing for sequencing of nicked, damaged, and short DNA fragments that may have otherwise been discarded with traditional library prep methods. The direct ligation of the adapters also eliminates the need for second strand synthesis, end repair, and dA tailing steps, thus reducing bias by preserving the integrity of any native methylation that is present on the fragment termini. Finally, the adapter-ligated cfDNA is indexed and amplified via PCR with Zymo-Seq UDIs, generating libraries that are ready for sequencing on any Illumina instrument.\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch4\u003e\u003cspan\u003e\u003cspan class=\"highlight\"\u003e\u003cspan\u003eSpecifics\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/h4\u003e\n\u003ctable cellpadding=\"0\" border=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eBarcode Sequences\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003ePlease refer to the Documents Section.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eBisulfite Conversion\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u0026gt;99.5% of non-methylated cytosine residues are converted to uracil; \u0026gt;99.5% protection of methylated cytosines.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eEquipment Required\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003eThermal cycler(s) with temperature adjustable lids, microcentrifuge, magnetic stand.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eHands-On Time\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e~2 hours\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eInput Quality\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003eFor optimal results, use at least minimum input of purified cfDNA with no RNA or gDNA contamination. cfDNA can be concentrated using the\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/www.zymoresearch.com\/products\/dna-clean-concentrator-5\"\u003eDNA Clean \u0026amp; Concentrator (D4013)\u003c\/a\u003e\u003cspan\u003e \u003c\/span\u003eprior to processing. Input cfDNA can be suspended in water, DNA Elution Buffer, or TE buffer.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eLibrary Storage\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003eLibraries eluted in DNA Elution Buffer (provided) may be stored at ≤ 4°C overnight or ≤ -20°C for long-term storage.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eMaximum Input\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e10 ng\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eMinimum Input\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e5 ng\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eSample Input Material\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003ePurified cell-free DNA (cfDNA)\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eSequencing Compatibility\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003eLibraries are compatible with all Illumina sequencing platforms. Recommended: NextSeq, NovaSeq.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003eTotal Processing Time\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e~6 hours\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003ca rel=\"noopener\" title=\"Zymo-Seq Cell Free DNA WGBS Library Kit Protocol Sheet\" href=\"https:\/\/files.zymoresearch.com\/protocols\/_d5462_d5463_zymo-seq_cell_free_dna_wgbs_library_kit.pdf\" target=\"_blank\"\u003e\u003cspan\u003e\u003cspan class=\"highlight\"\u003eProtocol \u003c\/span\u003e\u003c\/span\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca title=\"Zymo-Seq Cell Free DNA WGBS Library Kit FFPE Recommendations\" href=\"https:\/\/files.zymoresearch.com\/documents\/zymoseq_cell_free_dna_wgbs_library_kit__ffpe_dna_protocol_recommendations.pdf\"\u003eFFPE Recommendations\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca rel=\"noopener\" title=\"Zymo-Seq Cell Free DNA WGBS Library Kit Datasheet\" href=\"https:\/\/files.zymoresearch.com\/datasheets\/_ds2707_zymo-seq_cell_free_dna_wgbs_library_kit.pdf\" target=\"_blank\"\u003e\u003cspan\u003e\u003cspan class=\"highlight\"\u003eDatasheet \u003c\/span\u003e\u003c\/span\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan class=\"highlight\"\u003eSDS (MSDS): \u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca rel=\"noopener\" title=\"Zymo-Seq Cell Free DNA WGBS Library Kit (24 Preps) SDS\" href=\"https:\/\/files.zymoresearch.com\/sds\/_sdd5462_zymo-seq_cell_free_dna_wgbs_library_kit.pdf\" target=\"_blank\"\u003e\u003cspan\u003e\u003cspan class=\"highlight\"\u003eD5462\u003c\/span\u003e\u003c\/span\u003e\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca rel=\"noopener\" title=\"Zymo-Seq Cell Free DNA WGBS Library Kit (96 Preps) SDS\" href=\"https:\/\/files.zymoresearch.com\/sds\/_sdd5463_zymo-seq_cell_free_dna_wgbs_library_kit.pdf\" target=\"_blank\"\u003e\u003cspan\u003e\u003cspan class=\"highlight\"\u003eD5463\u003c\/span\u003e\u003c\/span\u003e\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Zymo Research","offers":[{"title":"1 EA \/ 24 Prep","offer_id":44880715579546,"sku":"D5462","price":1718.0,"currency_code":"USD","in_stock":false},{"title":"1 EA \/ 96 Prep","offer_id":44880715612314,"sku":"D5463","price":6590.0,"currency_code":"USD","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0593\/5025\/3722\/files\/D5462_Zymo-Seq-Cell-Free-DNA-WGBS-Library-Kit_600x_17529a9c-fe40-4950-aed9-ea1581f55b65.webp?v=1715786720"}],"url":"https:\/\/animal.ivfstore.com\/es\/collections\/kit.oembed","provider":"Animal IVF Store","version":"1.0","type":"link"}