Bovine Semen Wash Medium
For washing of semen during centrifugation prior to in vitro fertilization
Description and Prod. No.
50 ml non-capacitating medium in glass bottle for semen washing, Prod. No. 1.05.050
The medium contains a very low concentration of Sodium Bicarbonate and is phosphate buffered and must therefore not be CO
2 equilibrated
The medium is ready-to-use and does not need any supplements Medium should be preheated to 38.8°C prior to use with the lid on
Key Ingredients:
Calcium Chloride, Magnesium Sulphate, Potassium Chloride, Sodium Bicarbonate, Sodium Chloride, Sodium Dihydrogen Phosphate, Sodium Lactate, Sodium Pyruvate, Phenol Red and Gentamycin
Advantages of using a non-gradient and non-capacitating medium
- The capacitation of the spermatozoa is delayed until they are in the actual IVF medium with the oocytes
- Spermatozoa preserve energy for the subsequent capacitation, acrosome reaction and fertilization
- Potential toxic gradients and excessive centrifugation is avoided
- The Semen Wash Medium also applies very well to sex-sorted semen
Quality Control Tests and Manufacturing
- Sterility tested (Fungal and Bacterial)
- Osmolality tested and pH tested
- Endotoxin tested
- Manufacturer is ISO9001 and ISO13485 certified
- Controlled environment and to human standards
- Bovine Embryo Assay (BEA)
- Batch analysis certificate available
Storage:
- Store in original unopened container refrigerated 3-9 °C and protected from light
- Do not expose to temperatures higher than 39°C
- Do not freeze product it may precipitate < 2°C
- The medium must be used within 10 days after opening, providing it has been
- handled aseptically and is kept refrigerated after opening
- Discard unused medium if it has been warmed
