Botusemen Special is an extender specially developed for stallions more susceptible to cold damage. Its composition is based on adding essential lipids to maintain the plasma membrane stability during the cooling process.
To improve your results, use Botusemen Special during centrifugation in freezing protocols.
How to prepare
Add the sachet’s content to the sterile water and shake well.
How to use
The minimum dilution rate for preserving sperm motility during storage is 2 parts extender to 1 part semen, aiming for a final sperm concentration of 30 to 50 million total sperm per mL, as shown in the table below:
SPERM CONCENTRATION
DILUTION (V:V)
< 90 million sperm/ mL
Centrifuge or filter with Sperm Filter and resuspend 100 million sperm/mL
90 to 110 million sperm/ mL
2:1
120 to 130 million sperm/ mL
2,5:1
140 to 160 million sperm/ mL
3:1
170 to 180 million sperm/ mL
3,5:1
190 to 210 million sperm/ mL
4:1
220 to 230 million sperm/ mL
4,5:1
240 to 250 million sperm/ mL
5:1
IMPORTANT: In cases where centrifugation before the cooling process is necessary, resuspend the sperm pellet at a final concentration of 100 million total sperm per mL.
Unit - 200ml
One diluent bottle containing 200 mL of sterile water and 1 sachet with 17g.